英語翻譯綠色螢光蛋白（Green fluorescent protein,GFP）,最早是在水母中發現的一種發光蛋白.本

題目：

英語翻譯
綠色螢光蛋白（Green fluorescent protein,GFP）,最早是在水母中發現的一種發光蛋白.本實驗引用該基因並使其在大腸桿菌中進行表達、純化和鑑定.具體操作爲PCR擴增GFP基因,並將其插入到pTrcHis中,後將重組質粒轉化到E.coli（BL21菌株）中,使GEP得以表達,運用Western Blot和SDS-PAGE證明了綠色螢光蛋白在E.coli中的表達.

解答：

Green fluorescent protein (Green fluorescent protein,GFP),was first found in a jellyfish luminescent protein.The experimental application of the gene and its expression in E.coli,purification and identification.PCR amplification of specific operations for the GFP gene and insert it into the pTrcHis,will be transformed into E.coli recombinant plasmid (BL21 strain),the GEP expressed,using Western Blot and SDS-PAGE proved that green fluorescent protein in E.coli.